NMR Restraints Grid

Result table
 (Save to zip file containing files for each block)

image	mrblock_id	pdb_id	cing	stage	position	type
	6198	1iml	cing	1-original	1	comment

Experimental Restraints for the NMR Structures of the 
LIM Only protein Cysteine Rich Intestinal Protein, CRIP


The solution NMR 3D structure of the Cysteine Rich Intestinal Protein,
CRIP, is based on 500 distance restraints derived 
from the NOE data and 17 metal restraints. Hydrogen bond distance restraints
were not used for generation and refinement of the last 12 MODELS (MODEL 37-48).
A complete list of experimental restraints follows:

List of residues with corresponding numbering in the sequence
of the  48 MODELS:

	1  PKCPKCDKEV YFAERVTSLG
        21 KDWHRPCLKC EKCGKTLTSG
        41 GHAEHEGKPY CNHPCYSAMP
        61 GPKGFGRGGA ESHTFK

Pro-1  Lys-2  Cys-3  Pro-4  Lys-5  Cys-6  Asp-7  Lys-8  Glu-9  Val-10
Tyr-11 Phe-12 Ala-13 Glu-14 Arg-15 Val-16 Thr-17 Ser-18 Leu-19 Gly-20
Lys-21 Asp-22 Trp-23 His-24 Arg-25 Pro-26 Cys-27 Leu-28 Lys-29 Cys-30
Glu-31 Lys-32 Cys-33 Gly-34 Lys-35 Thr-36 Leu-37 Thr-38 Ser-39 Gly-40
Gly-41 His-42 Ala-43 Glu-44 His-45 Glu-46 Gly-47 Lys-48 Pro-49 Tyr-50
Cys-51 Asn-52 His-53 Pro-54 Cys-55 Tyr-56 Ser-57 Ala-58 Met-59 Phe-60
Gly-61 Pro-62 Lys-63 Gly-64 Phe-65 Gly-66 Arg-67 Gly-68 Gly-69 Ala-70
Glu-71 Ser-72 His-73 Thr-74 Phe-75 Lys-76

NOE Interproton distance restraints. Distances are in Angstroms (A)

Strong 1.8 - 2.7 A
Medium 1.8 - 3.3 A
Weak   1.8 - 5.0 A

Restraints to dummy atoms:

   + 0.5 for -CH3
   + 2.13 for cg-cd-hd of Tyr and Phe 
   + 2.13 for cz-ce-he of Tyr
   + 2.14 for cz-ce-he of Phe


Metal restraints used to enforce Zn-S and Zn-N bond distances,
and to ensure proper hybridization of the His-Nd and Cys-Sg atoms
(distances in A)

LB= Lower Bound
UB= Upper Bound


ATOM 1  ATOM 2  distance

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