NMR Restraints Grid
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NMR Restraints Grid |
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Result table
| image | mrblock_id | pdb_id | bmrb_id | cing | stage | program | type |
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610373 |
5t1o   |
30159 | cing | 2-parsed | STAR | comment |
data_5t1o_MR_file_constraints
save_Conversion_project
_Study_list.Sf_category study_list
_Study_list.Entry_ID parsed_5t1o
_Study_list.ID 1
loop_
_Study.ID
_Study.Name
_Study.Type
_Study.Details
_Study.Entry_ID
_Study.Study_list_ID
1 "Conversion project" NMR . parsed_5t1o 1
stop_
save_
save_entry_information
_Entry.Sf_category entry_information
_Entry.ID parsed_5t1o
_Entry.Title "Original constraint list(s)"
_Entry.Version_type original
_Entry.Submission_date .
_Entry.Accession_date .
_Entry.Last_release_date .
_Entry.Original_release_date .
_Entry.Origination .
_Entry.NMR_STAR_version 3.1
_Entry.Original_NMR_STAR_version .
_Entry.Experimental_method NMR
_Entry.Experimental_method_subtype .
loop_
_Related_entries.Database_name
_Related_entries.Database_accession_code
_Related_entries.Relationship
_Related_entries.Entry_ID
PDB 5t1o "Master copy" parsed_5t1o
stop_
save_
save_global_Org_file_characteristics
_Constraint_stat_list.Sf_category constraint_statistics
_Constraint_stat_list.Entry_ID parsed_5t1o
_Constraint_stat_list.ID 1
loop_
_Constraint_file.ID
_Constraint_file.Constraint_filename
_Constraint_file.Software_ID
_Constraint_file.Software_label
_Constraint_file.Software_name
_Constraint_file.Block_ID
_Constraint_file.Constraint_type
_Constraint_file.Constraint_subtype
_Constraint_file.Constraint_subsubtype
_Constraint_file.Constraint_number
_Constraint_file.Entry_ID
_Constraint_file.Constraint_stat_list_ID
1 5t1o.mr . . "MR format" 1 comment "Not applicable" "Not applicable" 0 parsed_5t1o 1
1 5t1o.mr . . XPLOR/CNS 2 distance NOE ambi 0 parsed_5t1o 1
1 5t1o.mr . . XPLOR/CNS 3 "dipolar coupling" "Not applicable" "Not applicable" 0 parsed_5t1o 1
1 5t1o.mr . . unknown 4 unknown "Not applicable" "Not applicable" 0 parsed_5t1o 1
1 5t1o.mr . . XPLOR/CNS 5 distance "hydrogen bond" ambi 0 parsed_5t1o 1
1 5t1o.mr . . unknown 6 "pseudocontact shift" "Not applicable" "Not applicable" 0 parsed_5t1o 1
1 5t1o.mr . . XPLOR/CNS 7 "dihedral angle" "Not applicable" "Not applicable" 0 parsed_5t1o 1
1 5t1o.mr . . "MR format" 8 "nomenclature mapping" "Not applicable" "Not applicable" 0 parsed_5t1o 1
stop_
save_
save_MR_file_comment_1
_Org_constr_file_comment.Sf_category org_constr_file_comment
_Org_constr_file_comment.Entry_ID parsed_5t1o
_Org_constr_file_comment.ID 1
_Org_constr_file_comment.Constraint_file_ID 1
_Org_constr_file_comment.Block_ID 1
_Org_constr_file_comment.Details "Generated by Wattos"
_Org_constr_file_comment.Comment
;
*HEADER TRANSFERASE 19-AUG-16 5T1O
*TITLE SOLUTION-STATE NMR AND SAXS STRUCTURAL ENSEMBLE OF NPR (1-85) IN
*TITLE 2 COMPLEX WITH EIN-NTR (170-424)
*COMPND MOL_ID: 1;
*COMPND 2 MOLECULE: PHOSPHOCARRIER PROTEIN NPR;
*COMPND 3 CHAIN: A;
*COMPND 4 SYNONYM: NITROGEN-RELATED HPR;
*COMPND 5 EC: 2.7.11.-;
*COMPND 6 ENGINEERED: YES;
*COMPND 7 MOL_ID: 2;
*COMPND 8 MOLECULE: PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE PTSP;
*COMPND 9 CHAIN: B;
*COMPND 10 SYNONYM: ENZYME I-NTR,PHOSPHOTRANSFERASE SYSTEM,ENZYME I;
*COMPND 11 EC: 2.7.3.9;
*COMPND 12 ENGINEERED: YES;
*COMPND 13 MUTATION: YES;
*COMPND 14 OTHER_DETAILS: THIS MOLECULE WAS MUTATED AT POSITION 356 FROM
*COMPND 15 HISTIDINE TO GLUTAMINE. IN E. COLI, WHEN THE NATIVE HISTIDINE IS
*COMPND 16 PRESENT, THE SAMPLE CAN BE PHOSPHORYLATED. IT WAS MUTATED TO PROVIDE
*COMPND 17 A HOMOGENEOUS SAMPLE. ADDITIONALLY, IT COMPRISES RESIDUES 170-424 OF
*COMPND 18 THE LARGER ENZYME I-NTR MOLECULE. RESIDUE 169 IS MUTATED TO GLYCINE
*COMPND 19 DUE TO TEV PROTEASE CLEAVAGE.
*SOURCE MOL_ID: 1;
*SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI O157:H7;
*SOURCE 3 ORGANISM_TAXID: 83334;
*SOURCE 4 GENE: PTSO, Z4569, ECS4085;
*SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
*SOURCE 6 EXPRESSION_SYSTEM_TAXID: 562;
*SOURCE 7 EXPRESSION_SYSTEM_STRAIN: ER2566;
*SOURCE 8 EXPRESSION_SYSTEM_PLASMID: PLASMID;
*SOURCE 9 MOL_ID: 2;
*SOURCE 10 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI (STRAIN K12);
*SOURCE 11 ORGANISM_TAXID: 83333;
*SOURCE 12 STRAIN: K12;
*SOURCE 13 GENE: PTSP, YGDF, YGDO, B2829, JW2797;
*SOURCE 14 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
*SOURCE 15 EXPRESSION_SYSTEM_TAXID: 562;
*SOURCE 16 EXPRESSION_SYSTEM_STRAIN: ER2566;
*SOURCE 17 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
*SOURCE 18 EXPRESSION_SYSTEM_PLASMID: PET28A
*KEYWDS PTSNTR, PHOSPHOTRANSFER, BACTERIAL, COMPLEX, TRANSFERASE
*EXPDTA SOLUTION NMR
*NUMMDL 20
*AUTHOR M.STRICKLAND,A.M.STANLEY,G.WANG,C.D.SCHWIETERS,S.BUCHANAN,
*AUTHOR 2 A.PETERKOFSKY,N.TJANDRA
*REVDAT 1 16-NOV-16 5T1O 0
;
save_
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