NMR Restraints Grid
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NMR Restraints Grid |
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Result table
| image | mrblock_id | pdb_id | bmrb_id | cing | stage | program | type |
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25798 |
2kc8   |
16065 | cing | 1-original | MR format | comment |
*HEADER TOXIN/TOXIN REPRESSOR 17-DEC-08 2KC8 *TITLE STRUCTURE OF E. COLI TOXIN RELE (R81A/R83A) MUTANT IN *TITLE 2 COMPLEX WITH ANTITOXIN RELBC (K47-L79) PEPTIDE *COMPND MOL_ID: 1; *COMPND 2 MOLECULE: TOXIN RELE; *COMPND 3 CHAIN: A; *COMPND 4 ENGINEERED: YES; *COMPND 5 MUTATION: YES; *COMPND 6 MOL_ID: 2; *COMPND 7 MOLECULE: ANTITOXIN RELB; *COMPND 8 CHAIN: B; *COMPND 9 FRAGMENT: UNP RESIDUES 47-79; *COMPND 10 ENGINEERED: YES *SOURCE MOL_ID: 1; *SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; *SOURCE 3 ORGANISM_TAXID: 83333; *SOURCE 4 STRAIN: K-12; *SOURCE 5 GENE: RELE, B1563, JW1555; *SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI; *SOURCE 7 EXPRESSION_SYSTEM_TAXID: 562; *SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21; *SOURCE 9 EXPRESSION_SYSTEM_VARIANT: DE3; *SOURCE 10 EXPRESSION_SYSTEM_VECTOR: PET28A; *SOURCE 11 OTHER_DETAILS: RELE IS EXPRESSED WITH HIS-TAG FUSION. THE *SOURCE 12 HIS-TAG IS REMOVED BY THROMBIN AFTERWARD; *SOURCE 13 MOL_ID: 2; *SOURCE 14 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; *SOURCE 15 ORGANISM_TAXID: 83333; *SOURCE 16 STRAIN: K-12; *SOURCE 17 GENE: RELB, B1564, JW1556; *SOURCE 18 EXPRESSION_SYSTEM: ESCHERICHIA COLI; *SOURCE 19 EXPRESSION_SYSTEM_TAXID: 562; *SOURCE 20 EXPRESSION_SYSTEM_STRAIN: BL21; *SOURCE 21 EXPRESSION_SYSTEM_VARIANT: DE3; *SOURCE 22 EXPRESSION_SYSTEM_VECTOR: PGEX2T; *SOURCE 23 OTHER_DETAILS: RELBC (K47-L79) IS EXPRESSED WITH GST-TAG *SOURCE 24 FUSION. THE GST-TAG IS REMOVED BY THROMBIN AFTERWARD *KEYWDS PROTEIN/PROTEIN COMPLEX, TOXIN RELE, ANTITOXIN RELB, *KEYWDS 2 REPRESSOR, STRESS RESPONSE, TOXIN, TRANSCRIPTION, *KEYWDS 3 TRANSCRIPTION REGULATION, TOXIN/TOXIN REPRESSOR COMPLEX *EXPDTA SOLUTION NMR *NUMMDL 20 *AUTHOR G.LI, Y.ZHANG, M.INOUYE, M.IKURA *REVDAT 1 17-MAR-09 2KC8 0
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