Result table
| image | mrblock_id | pdb_id | bmrb_id | cing | stage | program | type |
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473394 | 2k0x RC | 15658 | cing | 1-original | MR format | comment |
*HEADER TRANSPORT PROTEIN 15-FEB-08 2K0X *TITLE THE ACTINORHODIN HOLO ACYL CARRIER PROTEIN FROM S. *TITLE 2 COELICOLOR *COMPND MOL_ID: 1; *COMPND 2 MOLECULE: ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER *COMPND 3 PROTEIN; *COMPND 4 CHAIN: A; *COMPND 5 SYNONYM: ACP, ACTI ORF3; *COMPND 6 ENGINEERED: YES; *COMPND 7 MUTATION: YES *SOURCE MOL_ID: 1; *SOURCE 2 ORGANISM_SCIENTIFIC: STREPTOMYCES COELICOLOR; *SOURCE 3 STRAIN: A3(2); *SOURCE 4 GENE: ACTI; *SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI; *SOURCE 6 EXPRESSION_SYSTEM_STRAIN: BL21; *SOURCE 7 EXPRESSION_SYSTEM_VARIANT: DE3; *SOURCE 8 EXPRESSION_SYSTEM_VECTOR: PET11C; *SOURCE 9 OTHER_DETAILS: ACTINORHODIN ACYL CARRIER PROTEIN (ACT ACP) *SOURCE 10 FROM S. COELICOLOR WAS HETEROLOGOUSLY OVEREXPRESSED IN ITS *SOURCE 11 APO FORM IN E. COLI BL21 (DE3) CELLS. THESE CELLS *SOURCE 12 CONTAINED THE PLASMID PET11C C17S ACT ACP (COURTESY OF DR. *SOURCE 13 TOM NICHOLSON). THIS IPTG INDUCIBLE VECTOR IS BOTH EASIER *SOURCE 14 TO USE AND MORE RELIABLE THAN THE HEAT INDUCIBLE PT7-7 *SOURCE 15 VERSION ORIGINALLY CONSTRUCTED. THE PROTEIN WAS MODIFIED *SOURCE 16 TO THE HOLO FORM USING S. COELICOLOR ACPS AND ENZYME COA. *KEYWDS ACYL CARRIER PROTEIN, POLYKETIDE, HOLO, PHOSPHOPANTETHEINE, *KEYWDS 2 ANTIBIOTIC BIOSYNTHESIS, TRANSPORT PROTEIN *EXPDTA NMR, 20 STRUCTURES *AUTHOR M.P.CRUMP, S.E.EVANS, C.WILLIAMS *REVDAT 1 16-SEP-08 2K0X 0