BMRB

NMR Restraints Grid

Result table
 (Save to zip file containing files for each block)

image mrblock_id pdb_id bmrb_id cing stage position program type subtype subsubtype
144008 2kmj RC 16431 cing 1-original 1 HADDOCK distance NOE ambi


!
! HADDOCK AIR restraints for ligand to RNA
! based on NOEs
!
! Calibration        RNALIG-D2O   RNALIG-H2O    RNALIG-H2OB
! strong : 4.0 A     > 20.000     > 100.000     > 50.000
! medium : 5.0 A     > 10.000     >  50.000     > 20.000
! weak   : 6.0 A     < 10.000     <  50.000     < 20.000
! slight modifications due to violations (NOEs from residue G26 excluded???)
!
! For case of 2 ligand (B and C) to RNA (A)
!
!
!
! RNALIG-D2O: Peak 3    R#HD#-?        3.083    7.850      11129 
!
assign (resid 41 and name H6  and segid A)
       ((resid 1 or resid 3) and name HD# and segid C) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 6    R#HG2-C41H5    1.517    5.602       9875 
! RNALIG-D2O: Peak 7    R#HG1-C41H5    1.576    5.601      11861    (avg calib and assign both)
!
assign (resid 41 and name H5 and segid A)
       ((resid 1 or resid 3) and name HG2 and segid C) 5.5 5.5 0.0
assign (resid 41 and name H5 and segid A)
       ((resid 1 or resid 3) and name HG1 and segid C) 5.5 5.5 0.0
!
!
! RNALIG-D2O: Peak 8    R#HD#-A22H1'   3.082    5.884      17001 
!
assign (resid 22 and name H1' and segid A)
       ((resid 1 or resid 3) and name HD# and segid B) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 9    R#HD#-A22H8    3.081    7.670      49456 
! RNALIG-H2O: Peak 6    R#HD#-A22H8    3.076    7.663      77992 
! RNALIG-H2OB:Peak 65   R#HD#-A22H8    3.076    7.668      17259 
!
assign (resid 22 and name H8 and segid A)
       ((resid 1 or resid 3) and name HD# and segid B) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 10   R#HD#-U23H1'   3.082    5.783      22267 
!
assign (resid 23 and name H1' and segid A)
       ((resid 1 or resid 3) and name HD# and segid B) 4.0 4.0 0.0
!
!
! RNALIG-D2O: Peak 11   R#HD#-U23H5    3.082    5.458     108552 
! RNALIG-D2O: Peak 47   U23H5-R#HD#    5.458    3.084      86570 
! RNALIG-H2O: Peak 47   R#HD#-U23H5    3.071    5.453     110687 
! RNALIG-H2O: Peak 84   U23H5-R3HD#    5.448    3.075     234850
!
assign (resid 23 and name H5 and segid A)
       ((resid 1 or resid 3) and name HD# and segid B) 4.0 4.0 0.0
!
!
! RNALIG-D2O: Peak 12   R#HD#-U23H6    3.081    7.623      38543
! RNALIG-D2O: Peak 53   U23H6-R#HD#    7.622    3.078      36985   
! RNALIG-H2O: Peak 5    R#HD#-U23H6    3.074    7.616      68883 
!
assign (resid 23 and name H6 and segid A)
       ((resid 1 or resid 3) and name HD# and segid B) 4.0 4.0 0.0
!
!
! RNALIG-D2O: Peak 13   R#HD#-U23H2'   3.078    4.409      28353
!
assign (resid 23 and name H2' and segid A)
       ((resid 1 or resid 3) and name HD# and segid B) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 14   R#HD#-G26H1'   3.085    5.826      10117 
!
assign (resid 26 and name H1' and segid A)
       ((resid 1 or resid 3) and name HD# and segid B) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 15   R#HD#-A27H8    3.082    7.711      20571 
!
assign (resid 27 and name H8 and segid A)
       ((resid 1 or resid 3) and name HD# and segid B) 6.0 6.0 0.0
!
!
! RNALIG-D2O: Peak 16   R#HD#-C39H5    3.084    5.532      15381 
!
assign (resid 39 and name H5 and segid A)
       ((resid 1 or resid 3) and name HD# and segid C) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 17   R#HD#-U40H?    3.082    5.411      24482
!
assign (resid 40 and (name H5 or name H1') and segid A)
       ((resid 1 or resid 3) and name HD# and segid C) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 18   R#HD#-C41H5    3.082    5.603      15866 
!
assign (resid 41 and name H5 and segid A)
       ((resid 1 or resid 3) and name HD# and segid C) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 19   R#HD#-U42H5    3.083    5.098      29046 
!
assign (resid 42 and name H5 and segid A)
       ((resid 1 or resid 3) and name HD# and segid C) 4.0 4.0 0.0
!
!
! RNALIG-D2O: Peak 20   R#HG1-A22H1'   1.580    5.885       8165 
! RNALIG-D2O: Peak 26   R#HG2-A22H1'   1.519    5.885       8161    (avg calib and assign both)
!
assign (resid 22 and name H1' and segid A)
       ((resid 1 or resid 3) and name HG1 and segid B) 6.0 6.0 0.0
assign (resid 22 and name H1' and segid A)
       ((resid 1 or resid 3) and name HG2 and segid B) 6.0 6.0 0.0
!
!
! RNALIG-D2O: Peak 21   R#HG1-A22H8    1.579    7.670      18361 
! RNALIG-D2O: Peak 27   R#HG2-A22H8    1.520    7.671      16660    (avg calib and assign both)
!
assign (resid 22 and name H8 and segid A)
       ((resid 1 or resid 3) and name HG1 and segid B) 5.0 5.0 0.0
assign (resid 22 and name H8 and segid A)
       ((resid 1 or resid 3) and name HG2 and segid B) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 22   R#HG1-U23H1'   1.571    5.780       8438 
! RNALIG-D2O: Peak 28   R#HG2-U23H1'   1.518    5.783       9382    (avg calib and assign both)
!
assign (resid 23 and name H1' and segid A)
       ((resid 1 or resid 3) and name HG1 and segid B) 6.0 6.0 0.0
assign (resid 23 and name H1' and segid A)
       ((resid 1 or resid 3) and name HG2 and segid B) 6.0 6.0 0.0
!
!
! RNALIG-D2O: Peak 23   R#HG1-U23H2'   1.570    4.411      10298 
! RNALIG-D2O: Peak 29   R#HG2-U23H2'   1.514    4.411      12434    (avg calib and assign both)
!
assign (resid 23 and name H2' and segid A)
       ((resid 1 or resid 3) and name HG1 and segid B) 6.0 6.0 0.0
assign (resid 23 and name H2' and segid A)
       ((resid 1 or resid 3) and name HG2 and segid B) 6.0 6.0 0.0
!
!
! RNALIG-D2O: Peak 24   R#HG1-U23H5    1.573    5.459      56733 
! RNALIG-D2O: Peak 48   U23H5-R#HG1    5.457    1.580      31133 
! RNALIG-H2O: Peak 85   U23H5-R#HG1    5.453    1.557      66240 
! RNALIG-D2O: Peak 30   R#HG2-U23H5    1.520    5.459      66681 
! RNALIG-D2O: Peak 49   U23H5-R#HG2    5.458    1.521      32818 
! RNALIG-H2O: Peak 86   U23H5-R#HG2    5.448    1.510      62707 
! RNALIG-H2OB:Peak 64   R#HG2-U23H5    1.519    5.459      45110    (avg calib and assign both)
!
assign (resid 23 and name H5 and segid A)
       ((resid 1 or resid 3) and name HG1 and segid B) 4.0 4.0 0.0
assign (resid 23 and name H5 and segid A)
       ((resid 1 or resid 3) and name HG2 and segid B) 4.0 4.0 0.0
!
!
! RNALIG-D2O: Peak 25   R#HG1-U23H6    1.576    7.621      17503 
! RNALIG-D2O: Peak 54   U23H6-R#HG1    7.622    1.577      12352 
! RNALIG-D2O: Peak 31   R#HG2-U23H6    1.518    7.622      19677 
! RNALIG-D2O: Peak 55   U23H6-R#HG2    7.621    1.516      13512    (avg calib and assign both)
!
assign (resid 23 and name H6 and segid A)
       ((resid 1 or resid 3) and name HG1 and segid B) 5.0 5.0 0.0
assign (resid 23 and name H6 and segid A)
       ((resid 1 or resid 3) and name HG2 and segid B) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 32   R1HB#-A22H1'   1.859    5.882       6397 
!
assign (resid 22 and name H1' and segid A)
       (resid 1 and name HB# and segid B) 6.0 6.0 0.0
!
!
! RNALIG-D2O: Peak 33   R1HB#-A22H8    1.851    7.670      17709 
! RNALIG-D2O: Peak 46   A22H8-R1HB#    7.672    1.846       7812 
! RNALIG-H2O: Peak 3    R1HB#-?        1.842    7.662      69036 
!
assign (resid 22 and name H8 and segid A)
       (resid 1 and name HB# and segid B) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 34   R1HB#-U23H1'   1.847    5.788       8341 
!
assign (resid 23 and name H1' and segid A)
       (resid 1 and name HB# and segid B) 6.0 6.0 0.0
!
!
! RNALIG-D2O: Peak 35   R1HB#-U23H2'   1.849    4.410      19740 
!
assign (resid 23 and name H2' and segid A)
       (resid 1 and name HB# and segid B) 6.0 6.0 0.0
!
!
! RNALIG-D2O: Peak 36   R1HB#-U23H5    1.850    5.458      41122 
! RNALIG-D2O: Peak 50   U23H5-R1HB#    5.457    1.850      23672
! RNALIG-H2O: Peak 87   U23H5-R1HB#    5.444    1.843      37179 
!
assign (resid 23 and name H5 and segid A)
       (resid 1 and name HB# and segid B) 4.0 4.0 0.0
!
!
! RNALIG-D2O: Peak 37   R1HB#-U23H6    1.849    7.622      18553 
! RNALIG-D2O: Peak 56   U23H6-R1HB#    7.618    1.847       9181 
!
assign (resid 23 and name H6 and segid A)
       (resid 1 and name HB# and segid B) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 38   R#HD#-U42H6    3.083    7.740      16439 
!
assign (resid 42 and name H6 and segid A)
       ((resid 1 or resid 3) and name HD# and segid C) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 39   X2HT-G26H1'    7.293    5.827      14816 
!
!!!assign (resid 26 and name H1' and segid A)
!!!       (resid 2 and name HT and segid B) 5.0 5.0 0.0
!
!
! RNALIG-D2O: Peak 40   ?-U23H5        1.774    5.459      24662 
! RNALIG-D2O: Peak 52   U23H5-?        5.453    1.776      20960
! RNALIG-D2O: Peak 41   ?-U23H5        1.701    5.459      20455
! RNALIG-D2O: Peak 51   U23H5-?        5.456    1.721      11894    (avg calib and assign both)
!
assign (resid 23 and name H5 and segid A)
       ((resid 3 and name HB1 and segid B) or
       (resid 2 and (name HB1 or name HG1) and segid B)) 4.5 4.5 0.0
assign (resid 23 and name H5 and segid A)
       ((resid 3 and name HB2 and segid B) or
       (resid 2 and (name HB2 or name HG2) and segid B)) 4.5 4.5 0.0
!
!
! RNALIG-D2O: Peak 42   ?-U23H6        1.699    7.614       9354    (no stereo specific assign)
!
assign (resid 23 and name H6 and segid A)
       ((resid 3 and name HB# and segid B) or
       (resid 2 and (name HB# or name HG#) and segid B)) 6.0 6.0 0.0
!
!
! RNALIG-D2O: Peak 43   R3HB1-U23H6    1.767    7.623       9785 
! RNALIG-D2O: Peak 57   U23H6-R3HB1    7.621    1.777       7086    (no stereo specific assign)
!
assign (resid 23 and name H6 and segid A)
       ((resid 3 and name HB# and segid B) or
       (resid 2 and (name HB# or name HG#) and segid B)) 6.0 6.0 0.0
!
!
! RNALIG-D2O: Peak 44   R3HA-U23H1'    4.215    5.782      73993 
!
assign (resid 23 and name H1' and segid A)
       (resid 3 and name HA  and segid B) 4.0 4.0 0.0
!
!
! RNALIG-D2O: Peak 45   R3HA-U23H6     4.215    7.626      41425 
! RNALIG-D2O: Peak 58   U23H6-R3HA     7.620    4.217      41334 
!
assign (resid 23 and name H6 and segid A)
       (resid 3 and name HA and segid B) 4.0 4.0 0.0
!
!
! RNALIG-H2O: Peak 22   G26H8-R3HN     7.694    8.508      72012 
!
!!!assign (resid 26 and name H8 and segid A)
!!!       (resid 3 and name HN and segid B) 5.0 5.0 0.0
!
!
! RNALIG-H2O: Peak 23   G26H22-R3HN    7.978    8.507      66014 
!
!!!assign (resid 26 and name H22 and segid A)
!!!       (resid 3 and name HN and segid B) 5.0 5.0 0.0
!
!
! RNALIG-H2OB: Peak 56   R1HA-U40H6    4.016    7.939      19152 
!
assign (resid 40 and name H6 and segid A)
       (resid 1 and name HA and segid C) 6.0 6.0 0.0
!
!
! RNALIG-H2O: Peak new  R#HD#-C39H42   3.074    8.361      weak 
!
assign (resid 39 and name H42 and segid A)
       ((resid 1 or resid 3) and name HD# and segid B) 6.0 6.0 0.0
!
!
! aus 15N-HSQC-NOESY
!
assign (resid 26 and name H1 and segid A)
       ((resid 1 or resid 3) and name HD# and segid B) 6.0 6.0 0.0
!
!
! aus D2O NOESY
!
assign (resid 22 and name H2 and segid A)
       ((resid 1 or resid 3) and name HD# and segid B) 6.0 6.0 0.0