*HEADER    DNA                                     23-JAN-01   1HZ2              
*TITLE     SOLUTION NMR STRUCTURE OF SELF-COMPLEMENTARY DUPLEX 5'-               
*TITLE    2 D(AGGCG*CCT)2 CONTAINING A TRIMETHYLENE CROSSLINK AT THE N2          
*TITLE    3 POSITION OF G*. MODEL OF A MALONDIALDEHYDE CROSSLINK                 
*COMPND    MOL_ID: 1;                                                            
*COMPND   2 MOLECULE: DNA (5'-D(*AP*GP*GP*CP*(+G)P*CP*CP*T)-3');                 
*COMPND   3 CHAIN: A, B;                                                         
*COMPND   4 ENGINEERED: YES                                                      
*SOURCE    MOL_ID: 1;                                                            
*SOURCE   2 SYNTHETIC: YES                                                       
*KEYWDS    DNA DUPLEX, INTERSTRAND CROSSLINK                                     
*EXPDTA    NMR, MINIMIZED AVERAGE STRUCTURE                                      
*AUTHOR    P.A.DOOLEY, D.TSAROUHTSIS, G.A.KORBEL, L.V.NECHEV,                    
*AUTHOR   2 J.SHEARER, I.S.ZEGAR, C.M.HARRIS, M.P.STONE, T.M.HARRIS              
*REVDAT   1   07-FEB-01 1HZ2    0                                                
Distance Restraints Derived from NOE Data Using MARDIGRAS for the Crosslinked 5'-d(AGGCG*CCT)2 Oligodeoxynucleotide


Class 1 Distance Restraints






Residue 1
Atom
Residue 2
Atom
Distance
(?)
Lower Limit
Upper Limit
1
H1'
1
H4'
3.892
2.934
4.850
1
H8
1
H1'
2.736
2.436
3.036
1
H2''
1
H4'
2.331
2.031
2.631
1
H2''
1
H1'
2.434
2.134
2.734
1
H2''
1
H2'
1.925
1.625
2.225
1
H3'
1
H2'
3.019
2.690
3.349
2
H1'
2
H4'
3.018
2.718
3.318
2
H8
1
H2''
3.099
2.799
3.399
2
H8
2
H1'
3.691
3.391
3.991
2
H3'
2
H4'
2.769
2.469
3.069
3
H2'
3
H1'
2.879
2.579
3.179
3
H3'
3
H4'
2.677
2.377
2.977
4
H1'
4
H4'
2.735
2.435
3.035
4
H6
3
H1'
3.348
3.048
3.648
4
H2'
4
H6
2.258
1.958
2.558
4
H2''
4
H4'
2.699
2.399
2.999
4
H2''
4
H1'
2.365
2.065
2.665
4
H3'
4
H4'
2.672
2.372
2.972
4
H3'
4
H6
3.693
3.393
3.993
4
H3'
4
H2'
2.791
2.491
3.091
4
H3'
4
H2''
3.988
3.076
4.901
5
H1'
5
H4'
3.122
2.822
3.422
5
H8
4
H5
3.753
3.453
4.053
5
H2'
5
H1'
2.994
2.694
3.294
5
H2'
5
H8
2.426
2.126
2.726
5
H2''
5
H1'
2.430
2.130
2.730
5
H2''
5
H8
2.659
2.359
2.959
5
H3'
5
H4'
2.620
2.320
2.920
5
H3'
5
H2'
2.460
2.160
2.760
6
H6
5
H1'
3.122
2.822
3.422
6
H6
6
H4'
3.209
2.909
3.509
6
H5
5
H1'
3.443
3.143
3.743
7
H1'
7
H4'
3.045
2.745
3.345
7
H2'
7
H1'
3.493
2.351
4.635
7
H2''
7
H6
2.603
2.269
2.938
7
H3'
7
H4'
3.172
2.872
3.472
7
H3'
7
H6
2.993
2.693
3.293
7
H3'
7
H2''
3.558
3.186
3.930
8
M
8
H6
3.254
2.954
3.554
6
H5
5
H2'
3.948
3.365
4.532
6
H6
5
H2''
2.954
2.654
3.254
2
H1'
1
H4'
4.077
3.777
4.377
6
H6
5
H2'
4.255
3.807
4.703
4
H5
3
H2''
3.613
3.313
3.913
3
H2''
3
H8
2.347
2.047
2.647
6
H2'
6
H1'
2.818
2.110
3.526
3
H3'
3
H1'
3.955
3.485
4.425
4
H2'
4
H4'
3.973
3.217
4.730
6
H1'
6
H4'
2.447
2.147
2.747
7
H3'
7
H2'
2.782
2.482
3.082
6
H2'
6
H5
4.141
3.685
4.597
5
H8
4
H2'
3.380
3.080
3.680
4
H5
3
H8
3.638
3.338
3.938
2
H8
1
H2'
4.322
3.514
5.130
4
H2''
4
H2'
1.891
1.591
2.191
3
H2''
3
H1'
2.425
2.125
2.725
3
H3'
3
H2'
2.399
2.099
2.699
1
H2'
1
H8
2.895
2.073
3.717
4
H6
3
H2'
3.272
2.931
3.614
13
HX31
6
H1'
2.820
2.322
3.319
5
H8
4
H2''
3.798
3.479
4.117
2
H3'
2
H8
4.754
2.741
5.250
13
HX21
6
H1'
3.347
2.931
3.763
7
H6
6
H2'
2.852
2.378
3.326
6
H2'
6
H6
2.802
2.502
3.102
8
H3'
8
H6
3.971
3.086
4.857
1
H2'
1
H1'
3.635
2.975
4.296
6
H6
6
H1'
3.010
2.710
3.310
4
H2'
4
H1'
3.345
2.917
3.773
1
H3'
1
H4'
3.337
2.643
4.031
3
H2'
3
H8
2.267
1.967
2.567
6
H5
5
H2''
2.997
2.697
3.297
6
H2'
6
H4'
4.052
3.227
4.877
13
HX32
6
H1'
2.364
2.064
2.664
7
H6
7
H1'
3.918
3.523
4.314
8
H1'
8
H4'
3.697
3.348
4.047
3
H8
2
H1'
3.950
3.289
4.612
5
H3'
5
H1'
4.948
3.367
5.300
4
H6
3
H2''
2.807
2.507
3.107
7
H2''
7
H1'
2.392
2.092
2.692
8
H6
7
H2'
4.822
3.913
5.122
7
H2'
7
H4'
5.030
3.170
5.330
2
H3'
2
H1'
4.025
3.669
4.382
7
H2''
7
H4'
3.030
2.730
3.330
7
H2'
7
H6
2.402
2.102
2.702
5
H2''
5
H2'
1.952
1.652
2.252
8
H6
8
H1'
4.398
3.597
5.200

Class 2 Distance Restraints






Residue 1
Atom
Residue 2
Atom
Distance
(?)
Lower Limit
Upper Limit
1
H3'
1
H2''
3.390
2.980
3.801
5
H2''
5
H4'
4.286
3.614
4.959
4
H5
3
H1'
4.621
4.035
5.208
4
H6
4
H4'
4.104
3.367
4.842
6
H2''
6
H1'
2.246
1.946
2.546
3
H2''
3
H4'
4.800
2.786
5.100
8
H6
7
H2''
3.467
3.034
3.901
4
H5
3
H2'
3.410
3.039
3.782
6
H2''
6
H2'
1.971
1.671
2.271
7
H2'
7
H5
4.693
3.661
4.993
4
H2''
4
H6
4.008
2.713
5.008
7
H3'
7
H1'
4.839
3.957
5.122
5
H3'
5
H8
4.011
3.564
4.459
7
H2''
7
H2'
2.011
1.709
2.314
4
H6
4
H1'
3.609
3.291
3.928
7
H2''
7
H5
5.079
4.220
5.379
3
H2''
3
H2'
2.021
1.710
2.332
3
H3'
3
H8
3.830
3.530
4.130
3
H2'
3
H4'
4.554
3.610
5.054

Class 3 Distance Restraints






Residue 1
Atom
Residue 2
Atom
Distance
(?)
Lower Limit
Upper Limit
4
H3'
4
H1'
4.939
4.202
5.239
3
H8
3
H1'
4.122
3.249
4.996
1
H2''
1
H8
3.278
2.418
4.139
7
H5
6
H2'
2.757
1.967
3.548
8
M
7
H5
4.532
4.232
4.832
5
H8
5
H1'
4.490
2.783
5.090
7
H5
6
H6
4.269
3.419
5.119

Class 4 Distance Restraints






Residue 1
Atom
Residue 2
Atom
Distance
(?)
Lower Limit
Upper Limit
2
H4'
1
H1'
5.023
3.938
5.323
8
H3'
8
H4'
3.091
2.642
3.541
5
H2'
5
H4'
4.029
3.649
4.410
1
H3'
1
H1'
4.703
4.090
5.117
4
H6
3
H3'
4.585
3.736
5.035
8
H6
7
H1'
4.974
4.220
5.228

Class 5 Distance Restraints






Residue 1
Atom
Residue 2
Atom
Distance
(?)
Lower Limit
Upper Limit
4
H2'
4
H5
4.287
3.987
4.587
3
H8
2
H3'
4.157
3.857
4.457
1
H3'
1
H8
3.849
3.549
4.149
5
H8
4
H3'
5.058
4.256
5.514
7
H6
6
H1'
3.904
3.255
4.554
3
H8
3
H4'
5.060
3.726
5.294
4
H6
3
H8
5.034
4.704
5.365
2
H8
2
H4'
5.185
4.467
5.404
1
H8
1
H4'
4.479
4.179
4.779
6
H6
5
H3'
5.015
4.400
5.330
6
H3'
6
H6
3.243
2.943
3.543
4
H5
3
H3'
4.999
3.798
5.310
2
H8
1
H1'
4.507
3.535
5.480
6
H6
5
H8
4.230
3.769
4.692
8
H6
8
H4'
4.801
3.937
5.666
6
H5
5
H3'
5.111
4.811
5.411
2
H8
1
H3'
5.121
4.414
5.428
5
H8
5
H4'
5.053
2.853
5.453
8
M
7
H6
3.833
3.533
4.133
8
M
7
H3'
5.032
3.658
5.307
8
H6
7
H3'
5.099
4.364
5.334
8
M
7
H1'
5.106
4.806
5.406

Dooley, P. A., et al.

4



  Entry H atom name         Submitted Coord H atom name
    1   1H5*    A   1          H5'         A   1   5.548  -1.234  -3.181
    2   2H5*    A   1          H5''        A   1   5.455  -2.875  -3.853
    3    H4*    A   1           H4'        A   1   5.030  -2.906  -1.420
    4    H3*    A   1           H3'        A   1   2.797  -3.662  -3.210
    5    H1*    A   1           H1'        A   1   2.301  -1.879   0.316
    6    H8     A   1           H8         A   1   0.152  -3.291  -0.762
    7   1H6     A   1          H61         A   1  -2.724   1.287  -3.749
    8   2H6     A   1          H62         A   1  -2.972  -0.279  -3.010
    9    H2     A   1           H2         A   1   1.655   2.237  -3.354
   10    H5T    A   1           H5T        A   1   3.254  -1.090  -3.805
   11   1H2*    A   1          H2'         A   1   1.753  -4.389  -0.940
   12   2H2*    A   1          H2''        A   1   3.390  -3.993  -0.127
   13   1H5*    G   2          H5'         G   2   6.333  -5.477   0.276
   14   2H5*    G   2          H5''        G   2   5.472  -5.934   1.762
   15    H4*    G   2           H4'        G   2   6.250  -3.264   0.810
   16    H3*    G   2           H3'        G   2   6.212  -4.695   3.214
   17    H1*    G   2           H1'        G   2   4.401  -1.395   2.314
   18    H8     G   2           H8         G   2   2.358  -4.372   2.783
   19    H1     G   2           H1         G   2  -0.445   0.949   4.762
   20   1H2     G   2          H21         G   2   2.691   2.373   4.204
   21   2H2     G   2          H22         G   2   1.032   2.610   4.710
   22   1H2*    G   2          H2'         G   2   4.697  -3.479   4.352
   23   2H2*    G   2          H2''        G   2   5.694  -2.037   4.038
   24   1H5*    G   3          H5'         G   3   6.459   0.029   2.085
   25   2H5*    G   3          H5''        G   3   8.191   0.095   2.471
   26    H4*    G   3           H4'        G   3   7.130   2.284   2.779
   27    H3*    G   3           H3'        G   3   8.840   1.113   4.492
   28    H1*    G   3           H1'        G   3   5.306   2.384   6.071
   29    H8     G   3           H8         G   3   6.221  -1.458   5.665
   30    H1     G   3           H1         G   3   0.251  -0.101   7.456
   31   1H2     G   3          H21         G   3   1.258   3.226   7.128
   32   2H2     G   3          H22         G   3  -0.032   2.114   7.529
   33   1H2*    G   3          H2'         G   3   7.436   0.340   6.252
   34   2H2*    G   3          H2''        G   3   7.558   2.055   6.744
   35   1H5*    C   4          H5'         C   4   5.357   6.196   5.693
   36   2H5*    C   4          H5''        C   4   6.963   6.572   6.350
   37    H4*    C   4           H4'        C   4   5.118   6.347   8.068
   38    H3*    C   4           H3'        C   4   7.547   5.079   8.110
   39    H1*    C   4           H1'        C   4   3.715   4.525   9.477
   40   1H4     C   4          H41         C   4   4.480  -2.113   9.364
   41   2H4     C   4          H42         C   4   2.769  -1.829   9.589
   42    H5     C   4           H5         C   4   6.079  -0.428   9.133
   43    H6     C   4           H6         C   4   6.441   1.949   9.180
   44   1H2*    C   4          H2'         C   4   6.567   3.884  10.355
   45   2H2*    C   4          H2''        C   4   5.540   5.403  10.580
   46   1H5*   +G   5          H5'       PX5   5   6.349   9.876  12.606
   47   2H5*   +G   5          H5''      PX5   5   7.288   8.443  13.084
   48    H4*   +G   5           H4'      PX5   5   4.360   8.863  13.046
   49    H3*   +G   5           H3'      PX5   5   6.159   8.410  14.971
   50    H1*   +G   5           H1'      PX5   5   3.762   5.981  13.711
   51    H8    +G   5           H8       PX5   5   6.741   3.978  13.785
   52    H1    +G   5           H1       PX5   5   1.198   1.508  12.155
   53   1H2    +G   5          H2X       PX5   5   0.265   4.933  12.541
   54   1H2*   +G   5          H2'       PX5   5   6.644   6.186  14.368
   55   2H2*   +G   5          H2''      PX5   5   5.280   5.759  15.415
   56   1H5*    C   6          H5'         C   6   2.432   6.997  14.149
   57   2H5*    C   6          H5''        C   6   1.571   8.121  15.198
   58    H4*    C   6           H4'        C   6   0.112   6.522  13.712
   59    H3*    C   6           H3'        C   6  -0.405   7.225  16.381
   60    H1*    C   6           H1'        C   6  -0.496   4.076  14.569
   61   1H4     C   6          H41         C   6   4.699   1.492  17.315
   62   2H4     C   6          H42         C   6   3.645   0.103  17.173
   63    H5     C   6           H5         C   6   3.850   3.867  16.626
   64    H6     C   6           H6         C   6   2.101   5.089  15.889
   65   1H2*    C   6          H2'         C   6  -0.098   5.208  17.283
   66   2H2*    C   6          H2''        C   6  -1.614   4.753  16.429
   67   1H5*    C   7          H5'         C   7  -3.690   2.265  15.537
   68   2H5*    C   7          H5''        C   7  -4.910   3.550  15.567
   69    H4*    C   7           H4'        C   7  -5.290   2.238  17.555
   70    H3*    C   7           H3'        C   7  -3.922   4.977  17.846
   71    H1*    C   7           H1'        C   7  -3.832   1.351  20.038
   72   1H4     C   7          H41         C   7   2.460   3.043  21.375
   73   2H4     C   7          H42         C   7   2.034   1.724  22.442
   74    H5     C   7           H5         C   7   0.894   4.080  19.865
   75    H6     C   7           H6         C   7  -1.367   3.959  18.971
   76   1H2*    C   7          H2'         C   7  -3.339   4.301  20.299
   77   2H2*    C   7          H2''        C   7  -4.936   3.352  20.426
   78   1H5*    T   8          H5'         T   8  -7.081   1.843  20.394
   79   2H5*    T   8          H5''        T   8  -8.356   3.071  20.428
   80    H4*    T   8           H4'        T   8  -8.670   1.608  22.277
   81    H3*    T   8           H3'        T   8  -8.205   4.410  22.656
   82   1H2*    T   8          H2'         T   8  -6.652   4.416  24.320
   83   2H2*    T   8          H2''        T   8  -7.805   3.529  25.339
   84    H1*    T   8           H1'        T   8  -6.650   1.583  25.156
   85    H3     T   8           H3         T   8  -2.203   0.842  24.977
   86   1H5M    T   8          H51         T   8  -2.463   6.017  23.824
   87   2H5M    T   8          H52         T   8  -2.450   5.231  22.226
   88   3H5M    T   8          H53         T   8  -1.111   4.958  23.362
   89    H6     T   8           H6         T   8  -5.039   4.574  23.788
   90    H3T    T   8           H3T        T   8  -9.766   3.991  24.342
   91   1H5*    A   9          H5'         A   9  -0.636  -5.422  26.998
   92   2H5*    A   9          H5''        A   9   0.939  -5.731  27.759
   93    H4*    A   9           H4'        A   9   1.198  -5.362  25.328
   94    H3*    A   9           H3'        A   9   2.402  -3.364  27.150
   95    H1*    A   9           H1'        A   9   0.976  -2.474  23.547
   96    H8     A   9           H8         A   9   2.829  -0.740  24.690
   97   1H6     A   9          H61         A   9   0.622   3.017  26.892
   98   2H6     A   9          H62         A   9  -0.957   3.149  27.634
   99    H2     A   9           H2         A   9  -2.936  -0.871  27.205
  100    H5T    A   9           H5T        A   9  -0.256  -3.161  27.617
  101   1H2*    A   9          H2'         A   9   3.480  -2.572  24.915
  102   2H2*    A   9          H2''        A   9   2.719  -4.066  24.087
  103   1H5*    G  10          H5'         G  10   3.411  -7.293  23.805
  104   2H5*    G  10          H5''        G  10   4.134  -6.616  22.330
  105    H4*    G  10           H4'        G  10   1.317  -6.644  23.175
  106    H3*    G  10           H3'        G  10   2.810  -7.069  20.848
  107    H1*    G  10           H1'        G  10   0.059  -4.438  21.524
  108    H8     G  10           H8         G  10   3.479  -3.242  21.187
  109    H1     G  10           H1         G  10  -0.805   0.731  18.746
  110   1H2     G  10          H21         G  10  -2.807  -0.232  18.804
  111   2H2     G  10          H22         G  10  -3.051  -1.855  19.412
  112   1H2*    G  10          H2'         G  10   2.119  -5.316  19.629
  113   2H2*    G  10          H2''        G  10   0.447  -5.892  19.844
  114   1H5*    G  11          H5'         G  11  -1.629  -5.629  21.525
  115   2H5*    G  11          H5''        G  11  -2.141  -7.328  21.549
  116    H4*    G  11           H4'        G  11  -4.041  -5.994  20.936
  117    H3*    G  11           H3'        G  11  -3.190  -8.082  19.477
  118    H1*    G  11           H1'        G  11  -3.618  -4.553  17.434
  119    H8     G  11           H8         G  11  -0.102  -6.230  18.147
  120    H1     G  11           H1         G  11   0.047  -0.133  16.197
  121   1H2     G  11          H21         G  11  -2.031   0.632  15.972
  122   2H2     G  11          H22         G  11  -3.436  -0.355  16.308
  123   1H2*    G  11          H2'         G  11  -2.110  -7.083  17.609
  124   2H2*    G  11          H2''        G  11  -3.800  -6.888  17.052
  125   1H5*    C  12          H5'         C  12  -7.540  -3.929  17.723
  126   2H5*    C  12          H5''        C  12  -8.071  -5.485  17.049
  127    H4*    C  12           H4'        C  12  -7.306  -3.648  15.415
  128    H3*    C  12           H3'        C  12  -6.739  -6.331  15.376
  129    H1*    C  12           H1'        C  12  -5.081  -2.785  14.162
  130   1H4     C  12          H41         C  12   1.098  -5.320  14.553
  131   2H4     C  12          H42         C  12   1.255  -3.588  14.359
  132    H5     C  12           H5         C  12  -0.985  -6.437  14.632
  133    H6     C  12           H6         C  12  -3.382  -6.135  14.503
  134   1H2*    C  12          H2'         C  12  -5.200  -5.687  13.213
  135   2H2*    C  12          H2''        C  12  -6.363  -4.273  12.955
  136   1H5*   +G  13          H5'       GPX  13 -10.816  -3.992  10.680
  137   2H5*   +G  13          H5''      GPX  13  -9.594  -5.214  10.266
  138    H4*   +G  13           H4'      GPX  13  -9.380  -2.265  10.356
  139    H3*   +G  13           H3'      GPX  13  -9.274  -4.104   8.418
  140    H1*   +G  13           H1'      GPX  13  -6.438  -2.328   9.737
  141    H8    +G  13           H8       GPX  13  -5.129  -5.661   9.765
  142    H1    +G  13           H1       GPX  13  -1.139  -0.959  10.811
  143   1H2    +G  13          H2        GPX  13  -4.098   1.033  10.980
  144   1H2*   +G  13          H2'       GPX  13  -7.244  -5.090   9.103
  145   2H2*   +G  13          H2''      GPX  13  -6.487  -3.875   8.058
  146   1H5*    C  14          H5'         C  14  -7.107  -0.771   9.329
  147   2H5*    C  14          H5''        C  14  -7.892   0.361   8.230
  148    H4*    C  14           H4'        C  14  -6.026   1.311   9.840
  149    H3*    C  14           H3'        C  14  -6.487   2.074   7.189
  150    H1*    C  14           H1'        C  14  -3.479   1.299   9.054
  151   1H4     C  14          H41         C  14  -0.636  -3.694   6.490
  152   2H4     C  14          H42         C  14  -2.247  -4.335   6.253
  153    H5     C  14           H5         C  14  -4.328  -2.907   6.871
  154    H6     C  14           H6         C  14  -5.085  -0.919   7.628
  155   1H2*    C  14          H2'         C  14  -4.603   1.271   6.312
  156   2H2*    C  14          H2''        C  14  -3.792   2.595   7.218
  157   1H5*    C  15          H5'         C  15  -0.860   3.814   8.084
  158   2H5*    C  15          H5''        C  15  -1.738   5.346   8.229
  159    H4*    C  15           H4'        C  15  -0.481   5.553   6.195
  160    H3*    C  15           H3'        C  15  -3.480   4.948   5.950
  161    H1*    C  15           H1'        C  15  -0.070   4.129   3.574
  162   1H4     C  15          H41         C  15  -1.899  -1.338   0.930
  163   2H4     C  15          H42         C  15  -3.240  -1.504   2.040
  164    H5     C  15           H5         C  15  -3.810   0.173   3.684
  165    H6     C  15           H6         C  15  -3.141   2.302   4.649
  166   1H2*    C  15          H2'         C  15  -3.058   4.392   3.442
  167   2H2*    C  15          H2''        C  15  -1.751   5.720   3.357
  168   1H5*    T  16          H5'         T  16  -2.016   9.279   2.123
  169   2H5*    T  16          H5''        T  16  -2.500   7.713   1.450
  170    H4*    T  16           H4'        T  16   0.369   8.781   1.680
  171    H3*    T  16           H3'        T  16  -1.682   9.327  -0.211
  172   1H2*    T  16          H2'         T  16  -1.874   7.376  -1.341
  173   2H2*    T  16          H2''        T  16  -0.316   7.822  -2.092
  174    H1*    T  16           H1'        T  16   0.841   6.301  -0.885
  175    H3     T  16           H3         T  16  -0.287   2.041  -1.179
  176   1H5M    T  16          H51         T  16  -4.285   3.797   1.543
  177   2H5M    T  16          H52         T  16  -4.534   2.473   0.384
  178   3H5M    T  16          H53         T  16  -5.049   4.123  -0.033
  179    H6     T  16           H6         T  16  -2.833   6.022  -0.099
  180    H3T    T  16           H3T        T  16   0.045  10.111  -1.429
  181   1H1   TME   1          HX11      GPX  13  -1.534   1.518  10.206
  182   2H1   TME   1          HX12      GPX  13  -1.308   0.600  11.622
  183   1H2   TME   1          HX21      GPX  13  -2.658   2.323  12.879
  184   2H2   TME   1          HX22      GPX  13  -2.602   3.338  11.468
  185   1H3   TME   1          HX31      GPX  13  -0.144   3.244  11.486
  186   2H3   TME   1          HX32      GPX  13  -0.224   2.376  13.003